Column:

Bio-C18, 5 µm, 200 Å, 4.6 x 250 mm

Mobile phase:

A: 1%TEA, adjust to pH 2.8 with H₃PO₄;
B: MeOH : ACN = 70 : 30 ( v/v )

      Time(min):
                   B%:

0        5      15        20
50     50     70       70  

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 237 nm

Injection volume:

10 µL

Sample:

1. Amlodipine （氨氯地平）        2. Valsartan （缬沙坦）