Column:

Amethyst C18-P, 5 µm, 120 Å, 4.6 × 150 mm

Mobile phase:

A: 5.44 g/L KH₂PO₄ (pH 4.0)     B: ACN

Time(min):

0   7.5   8.5   9.0   13.0

B (%):

2   50    50    2       2

Flow rate:

0.8 mL/min

Temperature:

Ambient

Detection:

UV 215 nm

Injection volume:

10 µL

Sample:

Sulbactam sodium （舒巴坦钠）