Column:

Amethyst C18-P, 5 µm, 120 Å, 4.6 × 150 mm

Mobile phase:

A: Sodium pentanesulfonate 1.5 g/L + NH₄H₂PO₄ 3.5 g/L, adjust to pH 3.0 with H₃PO₄
B: MeOH

Time(min):
B%:

0      15      20
35     35      45

Flow rate:

1.0 mL/min

Temperature:

25 ^oC

Detection:

287 nm

Injection volume:

10 µL

Sample:

Lomefloxacin hydrochloride （盐酸洛美沙星）