Column:

GP-C18, 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:

A: 1.02% KH₂PO₄ ( pH 3.1 ) : MeOH : ACN = 75 : 5 : 20 ( v/v )
B: 1.02% KH₂PO₄ ( pH 3.1 ) : MeOH : ACN = 50 : 30 : 20 ( v/v )

      Time(min): 
                   B%:

0     5   15   25   30    45

20  20  60  60  100  100

Flow rate:

1.0 mL/min

Temperature:

Room temperature

Detection:

UV 280 nm

Injection volume:

20 µL

Sample:

0.1 mg/mL Atracurium besylate （苯磺酸阿曲库铵） in mobile phase A
1. S.S   2. R.S   3. R.R