Column:

GP-Phenyl, 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:

Methanol : 2.7 g/L KH₂PO₄ ( adjust pH to 3.0 by H₃PO₄ ) = 50 : 50 ( v/v )

Flow rate:

1.0 mL/min

Temperature:

60 ℃

Detection:

UV 215 nm

Injection volume:

25 µL

Sample:

0.5 mg/mL Celecoxib and impurity A and impurity B in 75% Methanol
1. Impurity A （杂质A） 
2. Celecoxib （塞来昔布）
3. Impurity B （杂质B）