Column:

Amethyst C18-H ( 5 µm, 4.6 x 250 mm )

Mobile phase:

10 mM K₂HPO₄ ( pH 7.0 by H₃PO₄ ) : Acetonitrle = 65 : 35 ( v/v ) ,

Flow rate:

1.0 mL/min

Temperature:

40 ℃,

Detection:

289 nm

Injection volume:

20 µL

Sample:

0.04 mg/mL Pantoprazole Sodium (泮托拉唑钠) in 0.001mol/L NaOH : Acetonitrle ( 1/1, v/v )