Column:

BR-C18, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

0.05 M KH₂PO₄ (adjust pH to 5.7 - 5.8 with 10% H₃PO₄) : CH₃OH = 30 : 70 (v/v)

Flow rate:

0.8 mL/min

Temperature:

Ambient

Detection:

UV 215 nm

Injection volume:

10 µL

Sample:

Clotrimazole solution
1. Clotrimazole （克霉唑）