Column:

Zenix SEC-300, 3 µm, 300 Å, 7.8 × 300 mm

Mobile phase:

150 mM Phosphate buffer, pH 7.0

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 214 nm

Injection Volume:

20 µL

Sample:

Recombinant human tumor necrosis factor receptor-Fc (rhTNFR-Fc)
1、rhTNFR Fc oligomer
2、rhTNFR Fc
3、Impurity