Column:

Zenix SEC-100, 3 µm, 100 Å, 7.8 x 300 mm

Mobile phase:

Phosphate solution ( 3.37 mL 85% H₃PO₄ + 7.1 g Na₂SO₄ + 400 mL H₂O, adjust pH to 6.7 by 30% NaOH ) → 500 mL        

Flow rate:

1.0 mL/min

Temperature:

23 ^oC

Detection:

UV 214 nm

Injection volume:

20 µL

Sample:

Gonal-F （果纳芬）(Recombinant Human Follitropin Alfa for Injection)