Column:

Amethyst C18-H , 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:

Phosphate buffer ( pH 2.5 ) : Acetonitrile = 86 : 14 ( v/v )

Flow rate:

0.8 mL/min

Temperature:

Room temperature

Detection:

UV 310 nm

Injection volume:

20 µL

Sample:

0.1 mg/L Huperzine A in 0.01 M Hydrochloric acid
1. Huperzine A （石杉碱甲A）