Column:

Proteomix SAX-NP5, 5 µm, 4.6 x 250 mm

Mobile phase:

A: 20 mM Tris, pH 8.0
B: A + 0.5 M NaCl   

      Time(min):
                   B%:

0        21       
0        70          

Flow rate:

0.5 mL/min

Temperature:

Room temperature

Detection:

UV 260 nm

Injection volume:

50 µL

Sample:

Oligonucleotide （寡聚核苷酸）