Column:

Proteomix SAX-NP5, 5 µm, 4.6 x 250 mm

Mobile phase:

A: 20 mM Tris, pH 8.0             
B: A + 0.5 M NaCl

      Time(min):
                   B%:

0        30       
0        100          

Flow rate:

0.5 mL/min

Temperature:

23 ℃

Detection:

UV 214 nm

Injection volume:

1 µL

Sample:

A_10-20, 10 µM each in water