Column:

Bio-C18, 5 µm, 200 Å, 4.6×150 mm

Mobile phase:

CH₃OH : Buffer (0.3mL/L H₃PO₄, 0.94 g/L Sodium Hexanesulfonate) = 24: 76 (v/v)

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 220 nm

Injection volume:

20 µL

Sample:

1. Cimetidine （西咪替丁）
2. Impurity（杂质）