Column:

GP-C18, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

A: 0.34% Tetrabutyl ammonium hydrogen sulfate
B: ACN

      Time(min):
                   B%:

0   10  15  17  22
5   50  50   5    5 

Flow rate:

1.0 mL/min

Temperature:

Room temperature

Detection:

UV 220 nm

Injection volume:

10 µL

Sample:

System suitability solution