Column:

Amethyst C18-H, 5 mm, 120 Å, 4.6 x 250 mm

Mobile phase:


Time(min):
B%:

A: 25 mM H₃PO₄ ( Adjusted pH to 3.0 by Triethylamine ) : ACN = 87 : 13 ( v/v )
B: A CN
0  10  20  30
0   0   50  50

Flow rate:

1.5 mL/min

Temperature:

25 ℃

Detection:

UV 278 nm

Injection volume:

20 µL

Sample:

0.15 mg/mL Norfloxacin （诺氟沙星） in mobile phase A