Column:

BR-C18, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

0.02 M KH₂PO₄ (pH 3.5 with H₃PO₄) : ACN =65 : 35 (v/v)

Flow rate:

1.0 mL/min

Temperature:

Room temperature

Detection:

UV 241 nm

Injection volume:

10 µL

Sample:

1 µg/mL Carvedilol reference solution（卡维地洛，对照溶液） in mobile phase