Column:

Sapphire-C18 ( 5µm, 100 Ǻ, 4.6 x 150 mm )

Mobile phase:

Sodium acetate buffer ( 1.5g anhydrous sodium acetate and 0.4 mL acetic acid in 1L of water, pH 5.2 by 1M NaOH ) : methanol : acetonitrile = 60 : 33 : 7 (v/v) 

Flow rate:

1 mL/min

Temperature:

45℃

Detection:

UV 239 nm

Injection volume:

10µL