Column:

Bio-C8(2) ( 8µm, 100 Å, 4.6 x 250 mm )

Mobile phase:

( dissolve 5.44 g KH₂PO₄ and 0.08 g sodium octanesulfonate in 780 mL water, adjust pH 2.7 using H₃PO₄ and dilute to 800 mL with water ) : methanol : ACN = 80 : 15 : 5 ( v/v )

Flow rate:

1.5 mL/min

Temperature:

40℃

Detection:

UV 240 nm

Injection volume:

10µL