Column:

Mono GPC-100, 5 µm, 100 Å, 7.8 x 300 mm

Mobile phase:

DMF + 30 mM LiBr

Flow rate:

0.8 mL/min

Temperature:

Room temperature

Detection:

UV 280 nm

Injection volume:

10 µL

Sample:

Red: 0.2 mg/mL Cefpodoxime Proxetil （头孢泊肟酯） in mobile phase

Blue: 0.2 mg/mL Cefpodoxime Proxetil ( heated at 100 ℃ for 30 min ) （加热破坏后的头孢泊肟酯） in mobile phase