Column:

HP-Amino, 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:

Phosphate buffer ( 600 mg KH₂PO₄ + 279 mg Na₂HPO₄ + 1 L H₂O ) : Acetonitrile = 30 : 70 ( v/v )

Flow rate:

1.5 mL/min

Temperature:

35 ℃

Detection:

UV 210 nm

Injection Volume:

10 µL

Sample:

System Suitability Solution : 0.5 mg/mL Acarbose（阿卡波糖） in water ( damaged by NaOH )

1. Alkaline degradation product （阿卡波糖碱降解物）

2. Acarbose （阿卡波糖）