Column:

Bio-C4, 5 µm, 300 Å, 4.6 × 250 mm

Mobile phase:

0.05 M Tris（adjust pH to 7.5 with 1 M HCl）: Propanol = 70.5 : 29.5 (v/v)

Flow rate:

0.5 mL/min

Temperature:

45 ^oC

Detection:

UV 220 nm

Injection Volume:

20 µL

Sample:

1. Recombinant human growth hormone dimer （重组人生长激素二聚体）
2. Recombinant human growth hormone monomer （重组人生长激素单体）