Column:

GP-Phenyl, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

A: ACN       B: 0.5%CH₃COOH

      Time(min):
                 B(%):

0     15    30    40 
90    80    80    70

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 350 nm

Injection Volume:

10 µL

Sample:

Flos Lonicerae Japonicae extract
1. Galuteolin （木犀草苷）