Column:

Polysulfonix-SCX, 120 Å, 5 µm, 4.6 x 250 mm

Mobile phase:

Acetonitrile : 0.2% Phosphoric acid ( adjust pH to 3.8 by Ammonia ) = 55 : 45 ( v/v )                  

Flow rate:

0.6 mL/min

Temperature:

Room temperature

Detection:

UV 215 nm

Injection volume:

10 µL

Sample:

1.  Arecoline hydrobromide （氢溴酸槟榔碱） (0.1 mg/mL)