Column:

HP-Amino, 5 µm, 120 Å, 4.6 x 300 mm

Mobile phase:

Acetonitrile : Water = 70 : 30 ( v/v )

Flow rate:

1.0 mL/min

Temperature:

45 ^oC

Detection:

RI ( 30 ℃ )

Injection volume:

10 µL

Sample:

Lactose stystem suitability solution ( 1 mg/mL Lactose and 1 mg/mL Sucrse in water )

1. Water （水）

2. Sucrse （蔗糖）

3. Lactose （乳糖）