Column:
|
Amethyst C18-H, 5 µm, 120 Å, 4.6 x 250 mm
|
Mobile phase:
|
A: 0.05 M Sodium Acetate
B: Acetonitrile : water = 50 : 50 ( v/v )
|
Time( min ):
|
0.01 0.5 11 18 25 30 32 55 55.01 100 |
B%:
|
37 32 32 50 67 69 95 95 37 37 |
Temperature: |
Room temperature
|
Flow rate:
|
0.8 mL/min
|
Detection:
|
UV 360 nm
|
Injection Volume:
|
10 µL
|
Sample:
|
DNFB derivatived amino acids(二硝基氟苯衍生化氨基酸)
|
|
1. Asp(门冬氨酸)
3. Ser(丝氨酸)
5. Arg(精氨酸)
7. Thr(苏氨酸)
9. Ala(丙氨酸)
11. Met(甲硫氨酸)
13. Leu(亮氨酸)
15. Cys(半胱氨酸)
17. Tyr(酪氨酸)
|
2. Glu(谷氨酸)
4. His(组氨酸)
6. Gly(甘氨酸)
8. Pro(脯氨酸)
10. Val(缬氨酸)
12. Ile(异亮氨酸)
14. Phe(苯丙氨酸)
16. Lys(赖氨酸) |